"Human-derived Lake Victoria Marburg virus strain Popp (Vero cell passage 7), from the original human outbreak, was cultured in Vero C1008 cells (ECCAC Cat. Number 85020206) "
"Briefly, a Collison nebulizer containing 10 ml MARV was used to generate aerosol particles of approximately 1–3 μm. The aerosol was conditioned in a modified Henderson apparatus (Druett 1969). Marmosets were placed in a bespoke exposure/plethysmograph tube (EMMS, Hampshire), and the head only was exposed for 10 min to a dynamic aerosol maintained at 50–55% relative humidity and 18–20 °C."
This work was supported by the Transformational Medical Technologies program contract HDTRA01-03-D-0001-0010 from the Department of Defense Chemical and Biological Defense program through the Defense Threat Reduction Agency (DTRA). The authors acknowledge the skills of P Pearce and A Phelps for telemetry implant surgery and the numerous staff within the Biomedical Sciences department of Dstl who assisted in the care and checking of animals throughout the studies. Particular thanks to M Brown, D Rogers, S Sangster and V Savage for technical assistance.